INTRA CYTOPLASMIC SPERM INJECTION

OVERVIEW:

For generations, there was little that doctor could do to help infertile couples especially where male factor was involved (disorders of sperm production). However in the last few years there has been major break-through in the treatment of male subfertility in the form of Intra cytoplasmic sperm injection (ICSI). Till recently solution of stubborn cases of male subfertility was adoption, now thay can be treated successfully by this method.

WHAT IS ICSI?

This is the technique in which sigle spermatozoa (male germs) of the husband is injected into the wife’s egg. Upto 3 eggs which are fertilized by ICSI are transferred to the female uterus (Embryo transfer) Pregnancy rate with ICSI in similar to IVF.

WHO NEEDS ICSI?

Couples with following problems can be helped with this technology:
1. Very low sperm count (oligospermia)
2. Very low sperm motility
3. Failed fertilization in conventional IVF
4. Obstructive azoospermia (absence of spermatozoa due to obstruction)
5. Repeated IUI failure

WHAT ARE THE CHANCES OF SUCCESS?

Chance of success are about 40% however in any given couple chances of success depend upon various factors among which quality of semen and egg of wife are important examples. The success rate of ICSI/IVF seems to be low but one should bear in mind that in absolutely normal couples the possibility of achieving a pregnancy in any given cycle is one in six to one in nine.

HOW MANY ATTEMPTS OF ICSI/IVF CAN BE MADE?

From medical point of view there in no limit on the number of attempts but one has to take into consideration emotional and financial stress involved in this treatment.

STEP BY STEP ICSI

1. Preliminary check up of husband & wife
2. Baseline hormone levels of wife
3. Drug treatment (injections) to encourage egg production & maturation
4. Ultra sound monitoring of treatment
a) To measure the growths of follicles
b) To adjust dose of drugs
c) Prevent serious side effects (ovarian hyper stimulation syndrome)
5. Monitoring is carried out:
• By trans-vaginal ultrasound scanning (TVS)
• By measuring hormones in blood
6. Egg pick up
7. Semen collection & processing.
8. ICSI:- single spermatozoa (male germs) is injected into the egg under special (ICSI) microscope with micromanipualotor. Egg collection, semen processing & sperm (male germs) injection is performed on the same day
9. Injected eggs are incubated in culture medium under special environment (Incubator)
10. Embryo transfer. After two to three days of spermatozoa (male germs) injection
if fertilization takes place then 2 – 3 fertilized eggs (embryos) are transferred into the uterus (womb). This procedure normally does not need any anaesthesia or sedation

USE OF DONOR’ S SPERM

We strictly use husband’s spermatozoa (male germs) and wife’s egg. Donor’s spermatozoa (male germs), egg and surrogacy facilites are not available in our clinic.

7